欣博盛QuantiCyto® Human Adiponectin ELISA kit(人脂聯(lián)素)試劑盒采用雙抗體夾心法:抗 Human Adiponectin 單抗包被于酶標(biāo)板上,標(biāo)本和標(biāo)準(zhǔn)品中的 Human Adiponectin 會(huì)與單抗結(jié)合,游離的成分被洗去。加入酶標(biāo)抗體,抗 Human Adiponectin 抗體與結(jié)合在單抗上的 Human Adiponectin 結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物,若反應(yīng)孔中有 Human Adiponectin ,辣根過(guò)氧化物酶會(huì)使無(wú)色的顯色劑現(xiàn)藍(lán)色,加終止液變黃。在450 nm處測(cè)OD值, Human Adiponectin 濃度與OD450值之間呈正比,可通過(guò)繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中 Human Adiponectin 的濃度。
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